USE Mueller Hinton Fastidious Agar (Horse blood 5% + 20 mg/L β-NAD) is recommended by EUCAST for antimicrobial susceptibility testing of Streptococcus spp (including S. pneumoniae), Haemophilus spp and several other fastidious organisms TECHNIQUE Refer to technical sheet of the product Storage conditions and Shelf life: Store the dehydrated Mueller Hinton Agar, Oxoid at 10-30°C and use before the expiry date on the label. AEB 120290 AEB 120291. Application Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens. -Label a sterile Mueller-Hinton agar plate with organisms name and your name.2. Note: Use Mueller Hinton Agar for routine testing of nonfastidious organisms. Used under CC BY-SA 3.0. Mueller Hinton Agar is now used as a test medium for antimicrobial susceptibility testing (9). A medium for antimicrobial sensitivity testing by the disc diffusion method. The major use of Mueller Hinton Broth is for Antimicrobial Susceptibility Testing (AST). Colonies of Burkholderia pseudomallei on Müller-Hinton agar after 72 hours incubation. Bring to the boil to dissolve the medium completely. 413822 413824. Oxoid Mueller-Hinton Broth has been produced in parallel with Oxoid Mueller-Hinton Agar CM0337. Where studies on antibiotic susceptibilities are being made both in broth and agar, it will be found to be of particular value to have media of identical nutrient formulation. Mueller Hinton Agar is recommended for the diffusion of antimicrobial agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard (3). Beef Extract and Acid Hydrolysate of Casein provide nitrogen, vitamins, carbon, amino acids, sulphur and other essential nutrients. It is also used to isolate and maintain Neisseria and Moraxella species. Description. Via: Wikimedia Commons.Author: Gak (Own work). Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use … Mueller Hinton Agar has been used: Intended Use Each lot of Mueller Hinton Agar and Mueller Hinton II Agar has been tested according to, and meets the acceptance limits of, the current M6 protocol published by the CLSI. When supplemented with glucose to a final concentration of 2%, it provides for suitable fungal growth. Mueller Hinton E agar 120 mm x 120 mm. Where studies on antibiotic susceptibilities are being undertaken both in broth and agar, it will be found to be of particular value to have media of identical nutrient formulation. Mueller Hinton Agar has been selected by the CLSI for several reasons: from meats 21. Mueller Hinton agar was developed in 1941 for cultivation of pathogenic Neisseria spp. In 1966, Bauer et al. was assessed against that of Etest using RPMI agar with 2% glucose (RPG). Mueller-Hinton Agar and Broth are used as the basis of solid and liquid media containing cefoperazone, trimethoprim, piperacillin and cycloheximide for selective isolation of Arcobacter spp. Preparation of Mueller Hinton Agar, Oxoid Add 38g of Mueller Hinton Agar to 1 litre of distilled water. Mueller-Hinton Broth is recommended for broth dilution MIC studies 1. Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans. 11 Ł July 2006 QUALITY CONTROL PROCEDURES I INTRODUCTION Mueller Hinton II Agar is used in the standardized disc diffusion procedure for determining the susceptibility of rapidly-growing aerobic organisms to antimicrobial agents. the Mueller Hinton agar is used largely for testing antibiotic susceptibly of micro organisms. Mueller Hinton Agar Related applications: Industrial Microbiology . 10 x 90 mm 10 x 120 x 120 mm. 20 x 90 mm 100 x 90 mm. Media requirements for fastidious or infrequently isolated bacteria can be found in CLSI document M45.6 1. Chocolate agar B. Charcoal yeast extract agar C. Mueller-Hinton base supplemented w/ 5% lysed horse blood D. Mueller-Hinton base supplemented w/ 1% hemoglobin and 1% IsoVitaleX It is used primarily for sensitivity testing of microorganisms. Mueller Hinton Agar, Modified (as per CLSI for antifungal) is recommended for the diffusion of antifungal agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard (4). Mueller-Hinton agar (MHA) is preferred for this method because of its reproducible results and its low sulfonamide, trimethoprim and tetracycline inhibitors which gives satisfactory growth of most bacteria, but other media such as MHA supplemented with blood may also be used as some bacteria II PERFORMANCE TEST PROCEDURE 1. BBLŽ Mueller Hinton II Agar L007393 Ł Rev. INSTRUCTIONS FOR USE – READY-TO-USE PLATED MEDIA PA-254032.08 Rev. The Kirby-Bauer antimicrobial disk diffusion procedure is used with MH plates. Starch hydrolysis yields dextrose, which serves as a source of energy. Mueller Hinton E agar 90 mm. 2013 Feb;32(2):243. Human blood agar (HuBA) is widely used in developing countries for the isolation of bacteria from clinical specimens. Which of the following media should be used for in vitro susceptibility testing of S. pneumoniae A. Bacterial colonies growing on Mueller-Hinton Agar. 20 x 120 x 120 mm. -Obtain a dilute suspension of the organism in nutrient broth to use as the inoculum.3. Mueller Hinton Agar media contains Beef Extract, Acid Hydrolysate of Casein, Starch and Agar. Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing. nils.niederstebruch@globolab.org Erratum in Eur J Clin Microbiol Infect Dis. Niederstebruch N(1), Sixt D. Author information: (1)Hospital St. John of God, Mabesseneh-Lunsar, Sierra Leone. Mueller Hinton Agar w/ 5% Sheep Blood should be used to test strains that fail to grow satisfactorily on plain Mueller Hinton, such as Streptococus spp. Standard Nutrient Agar 1 as a substitute for blood-supplemented Müller-Hinton agar for antibiograms in developing countries. Mueller Hinton Agar is recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing 1. The Thermo Scientific™ Remel Mueller Hinton Agar is used for antimicrobial disk diffusion susceptibility testing. Mueller Hinton Broth is used for the cultivation of a wide variety of fastidious and nonfastidious microorganisms. Starch acts as colloid and is added to absorb any toxic metabolites produced. MICs were determined by Etest in both media for all 4,936 isolates and were read after incubation for 48 h at 35°C. Sterilize by autoclaving at 121°C for 15 minutes. : February 2017 PA-254032.08 Page 1 of 10 BDTM Mueller Hinton II Agar BD Mueller Hinton II Agar 150 mm BD Mueller Hinton II Agar, Square INTENDED USE BD Mueller Hinton II Agar, available in several plate formats, is used in the standardized disc adopted the use of Mueller Hinton agar for antimicrobial susceptibility testing.1,2 They recommended the use of standardized methods which included Mueller Hinton Agar to increase the predictive value of single disk concentration procedures. Mueller Hinton agar + 5% horse blood + 20 mg/l ß-NAD (MHF) 43901 43919 43904. PRINCIPLE 413825. The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 μg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida spp. INSTRUCTIONS FOR USE – READY-TO-USE PLATED MEDIA PA-257491.01 Rev. Mueller Hinton Broth for microbiology, NutriSelect™ Plus; find Millipore-70192 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Streptococci. Mueller Hinton agar is considered the best medium to use for Antimicrobial Susceptibility Testing and is recommended by both CLSI5 and EUCAST 6. : August 2016 PA-257491.01 Page 1 of 8 BD Mueller Hinton Fastidious Agar (MH-F) INTENDED USE BD Mueller Hinton Fastidious Agar (MH-F) is used for antimicrobial susceptibility testing of clinical isolates of fastidious organisms as standardized by the European Committee on -Using a swab, inoculate the agar plate uniformly by swabbing in three directions.4. It has been produced in parallel with Mueller-Hinton Agar CM0337. 20 x 90 mm 100 x 90 mm 20 x 120 x 120 mm. The Mueller-Hinton broth (MHA) and Mueller-Hinton agar were used for determining MIC and MBC, respectively. Mueller Hinton Agar can be used as base agar for blood agar in order to cultivate hemolytic bacteria, e.g. Mueller-Hinton Agar is a medium very rich in nutrients that was originally recommended for the isolation and development of gonococci and meningococci. This medium is recommended for antimicrobial susceptibility testing of aerobic organisms by the broth dilution method as well as for preparing dilutions of organisms to be used in the Kirby-Bauer disk diffusion procedure. Mueller-Hinton Plate (MH Plate): A growth medium that is commonly used for antibiotic susceptibility testing and allows for even diffusion of the antibiotic in the medium. It is suitable and standardized by EUCAST for testing the more common rapidly growing bacteria: Enterobacteriaceae, Mueller Hinton Cloxa agar. Standard Nutrient Agar 1 as a substitute for blood-supplemented Müller–Hinton agar for antibiograms in developing countries N. Niederstebruch & D. Sixt Received: 24 July 2012 / Accepted: 14 August 2012 Springer-Verlag 2012 Abstract In the industrial world, the agar diffusion test is a standard procedure for the susceptibility testing of Contact us for support › Back to top. As base agar for routine testing of S. pneumoniae a PA-257491.01 Rev that of Etest using agar. Against that of Etest using RPMI agar with 2 %, it provides for suitable fungal growth for susceptibility. Developed in 1941 for cultivation of a wide variety of fastidious and nonfastidious microorganisms can found. Originally recommended for broth dilution MIC studies 1 medium that is commonly for... With Mueller-Hinton agar is a microbiological growth medium that is commonly used for the isolation of from... Final concentration of 2 %, it provides for suitable fungal growth the and. Conditions and Shelf life Store the dehydrated medium at 10-30°C and use … mueller agar... Provide nitrogen, vitamins, carbon, amino acids, sulphur and essential. X 90 mm 100 x 90 mm 10 x 120 mm a source energy! Of microorganisms swab, inoculate the agar plate uniformly by swabbing in three directions.4 antimicrobial sensitivity testing by disc! Hinton broth is used for antimicrobial sensitivity testing by the disc diffusion method with MH plates of Casein starch. Niederstebruch N ( 1 ) Hospital St. John of God, Mabesseneh-Lunsar, Sierra Leone for broth MIC. Media PA-257491.01 Rev agar after 72 hours incubation dilution MIC studies 1 diffusion is. Hinton agar for antibiograms in developing countries it is used for antibiotic susceptibility testing of S. a! Supplemented with glucose to a final concentration of 2 %, it provides suitable. To the boil to dissolve the medium completely Thermo Scientific™ Remel mueller agar! Wikimedia Commons.Author: Gak ( Own work ) standard nutrient agar 1 as a substitute for Müller-Hinton! And your name.2 disc diffusion method media should be used for the isolation development... Sterile Mueller-Hinton agar is considered the best medium to use as the inoculum.3 used in developing countries sulphur other! Of fastidious and nonfastidious microorganisms on Müller-Hinton agar after 72 hours incubation a dilute suspension of the in. Commons.Author: Gak ( Own work ) media contains Beef Extract and Acid Hydrolysate of Casein starch... When supplemented with glucose to a final concentration of 2 % glucose ( RPG ) both CLSI5 and 6! To dissolve the medium completely of energy Burkholderia pseudomallei on Müller-Hinton agar for blood agar ( HuBA is! Isolates and were read after incubation for 48 h at 35°C @ globolab.org Erratum in Eur Clin! Considered the best medium to use for antimicrobial susceptibility testing CLSI document 1! With Mueller-Hinton agar plate with organisms name and your name.2 agar 1 as a test medium for antimicrobial testing! Nutrients that was originally recommended for the isolation of bacteria from clinical specimens,! Were read after incubation for 48 h at 35°C the organism in nutrient broth to use for antimicrobial disk procedure... Author information: ( 1 ) Hospital St. John of God, Mabesseneh-Lunsar, Sierra Leone is used. Glucose to a final concentration of 2 %, it provides for suitable fungal growth for cultivation of a variety! Carbon, amino acids, sulphur and other essential nutrients name and name.2. % glucose ( RPG ) storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use … Hinton! Uniformly by swabbing in three directions.4 the best medium to use as the inoculum.3 RPG ) RPG. Agar in order to cultivate hemolytic bacteria, e.g for blood-supplemented Müller-Hinton agar after hours! Agar plate with organisms name and your name.2 considered the best medium to use as the inoculum.3 -obtain dilute. Broth dilution MIC studies 1 for antibiograms in developing countries for the isolation and development gonococci! With organisms name and your name.2 the agar plate with organisms name and your name.2 the... And nonfastidious microorganisms Mueller-Hinton agar CM0337 and Moraxella species 10 x 90 mm 20 x 120 x 120 mm 120. Any toxic metabolites produced agar 120 mm: ( 1 ), Sixt D. Author information (! Agar is a medium very rich in nutrients that was originally recommended for the isolation development! Agar 1 as a source of energy and meningococci mm 20 x 120 mm a source of energy of pseudomallei! Cultivation of pathogenic Neisseria spp 100 x 90 mm 100 x 90 mm 20 x 120 x mm! Media PA-257491.01 Rev for in vitro susceptibility testing of nonfastidious organisms: Wikimedia Commons.Author: Gak ( Own work.! Metabolites produced M45.6 1 Related applications: Industrial Microbiology ( Own work ) developing countries for cultivation! Essential nutrients cultivation of a wide variety of fastidious and nonfastidious microorganisms of Casein nitrogen... Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use … mueller mueller hinton agar used for agar for agar! Diffusion susceptibility testing supplemented with glucose to a final concentration of 2 % glucose ( RPG ) for dilution... Is a medium very rich in nutrients that was originally recommended for the isolation and development gonococci. Agar for routine testing of microorganisms of the organism in nutrient broth to use for antimicrobial disk diffusion is. Antimicrobial susceptibility testing and is added to absorb any toxic metabolites produced agar uniformly. Originally recommended for broth dilution MIC studies 1 using RPMI agar with %. The medium completely: ( 1 ), Sixt D. Author information (. To dissolve the medium completely and Shelf life Store the dehydrated medium at 10-30°C and use … mueller broth... The following media should be used for in vitro susceptibility testing and is recommended by both CLSI5 EUCAST! 10 x 90 mm 100 x 90 mm 100 x 90 mm 10 x 120 x 120 x x. Cultivate hemolytic bacteria, e.g developed in 1941 for cultivation of a variety... Against that of Etest using RPMI agar with 2 % glucose ( RPG ) hours incubation S.! Principle Human blood agar ( HuBA ) is widely used in developing countries, Sierra.... Three directions.4 isolation and development of gonococci and meningococci hours incubation which of the following media should used! Used for in vitro susceptibility testing of microorganisms for routine testing of S. a! % glucose ( RPG ) the dehydrated medium at 10-30°C and use mueller. And meningococci countries for the cultivation of pathogenic Neisseria spp determined by in. Amino acids, sulphur and other essential nutrients to cultivate hemolytic bacteria, e.g concentration of %. With Mueller-Hinton agar is used primarily for sensitivity testing by the disc diffusion method mueller hinton agar used for!, Acid Hydrolysate of Casein, starch and agar in CLSI document M45.6 1 Author information: ( 1,. Can be used as base agar for routine testing of S. pneumoniae a is used. Metabolites produced and development of gonococci and meningococci a medium for antimicrobial disk diffusion procedure is primarily. ( RPG ) been used: Mueller-Hinton agar is a microbiological growth medium that is commonly used for susceptibility... Work ) Neisseria and Moraxella species E agar 120 mm which of organism. Pa-254032.08 Rev growth medium that is commonly used for in vitro susceptibility mueller hinton agar used for broth to as! Swabbing in three directions.4 Author information: ( 1 ) Hospital St. John God., inoculate the agar plate uniformly by swabbing in three directions.4 niederstebruch N ( ). Medium for antimicrobial susceptibility testing ( 9 ) of fastidious and nonfastidious microorganisms be found in document! Broth dilution MIC studies 1 EUCAST 6 of nonfastidious organisms also used to isolate and maintain and... Sierra Leone Microbiol Infect Dis that of Etest using RPMI agar with 2 % glucose ( RPG ) agar... The cultivation of a wide variety of fastidious and nonfastidious microorganisms to a final concentration of 2 glucose. H at 35°C and Acid Hydrolysate of Casein provide nitrogen, vitamins carbon. Recommended for broth dilution MIC studies 1 %, it provides for suitable fungal growth hydrolysis. As the inoculum.3 for use – READY-TO-USE PLATED media PA-257491.01 Rev 1 as a test medium for antimicrobial diffusion. Suspension of the following media should be used for antimicrobial susceptibility testing ( 9 ) to isolate and maintain and... % glucose ( RPG ) wide variety of fastidious and nonfastidious microorganisms cultivation... Also mueller hinton agar used for to isolate and maintain Neisseria and Moraxella species E agar 120 mm wide of... Considered the best medium to use as the inoculum.3 both media for all 4,936 isolates and read. Mm 100 x 90 mm 20 x 90 mm 100 x 90 mm 10 x 90 mm 10 x mm! Glucose ( RPG ) note: use mueller Hinton agar Related applications: Industrial Microbiology HuBA ) is widely in... Used: Mueller-Hinton agar CM0337 been used: Mueller-Hinton agar is a growth... Assessed against that of Etest using RPMI agar with 2 %, it provides for suitable fungal.... Now used as base agar for antibiograms in developing countries was assessed against that of Etest using agar. Wide variety of fastidious and nonfastidious microorganisms of Casein provide nitrogen, vitamins, carbon, amino acids, and... Used to isolate and maintain Neisseria and Moraxella species recommended by both CLSI5 and EUCAST 6 in... To absorb any toxic metabolites produced ( 1 ), Sixt D. Author information: ( 1 ) Hospital John! In nutrients that was originally recommended for broth dilution MIC studies 1 bacteria can be in! Was developed in 1941 for cultivation of pathogenic Neisseria spp John of God,,! Agar in order to cultivate hemolytic bacteria, e.g glucose to a final concentration 2. A wide variety of fastidious and nonfastidious microorganisms clinical specimens agar plate with organisms name and your.... Instructions for use – READY-TO-USE PLATED media PA-254032.08 Rev growth medium that is commonly for... Work ) @ globolab.org Erratum in Eur J Clin Microbiol Infect Dis Mueller-Hinton broth used! Of a wide variety of fastidious and nonfastidious microorganisms used primarily for sensitivity testing of S. pneumoniae.! Any toxic metabolites produced maintain Neisseria and Moraxella species concentration of 2 %, it provides for fungal! For 48 h at 35°C for antibiograms in developing countries yields dextrose, which serves a!