COVID-19 is an emerging, rapidly evolving situation. The rank order of biofilm-producing ability was S. aureus > S. pyogenes > E. coli > P. aeruginosa > K. pneumoniae. They comprised 43% men and 57% women aged between 21 and 80 years. -, Gilani AH, Atta-ur-Rahman. eCollection 2017. S. aureus was therefore selected for the investigation of biofilm formation inhibitory property of H. floribunda. Solanum surattense, Withania somnifera, Cyperus rotundus, Solanum nigrum and Melia azedarach were the most utilized medicinal plant species with highest used value. 2020 Nov 25;16(1):72. doi: 10.1186/s13002-020-00424-x. 2020 Dec 14;6(12):e05562. The plants were distributed across 63 genera belonging to 31 families, mostly Fabaceae (9), Euphorbiaceae (6), Moraceae (6), and Meliaceae (6). Demographic features of informants; life form, part used, methods of preparation, modes of application and ethnomedicinal uses were documented. Many Caribbean indigenous plants now identified solely as food crops were also valued by my ancestors for their medicinal properties. In terms of medicines, many different parts of plants were used. 1997). Medicinal plant species with high RPL values should be screened for comprehensive phytochemical and pharmacological studies. A total of 85 species belonging to 71 genera and 34 families were documented along with ethnomedicinal uses. Copyright © 2020 Elikplim Kwesi Ampofo et al. Ciprofloxacin was used as the reference drug. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. and Marak, P.N. doi: Cox PA.Will tribal knowledge survive the millennium? eCollection 2020. The activity of the individual isolated compounds was lower than the crude alkaloid fraction, suggesting the possible role of synergism in the activity of the steroidal alkaloids. Zones of growth inhibition were determined after incubation. HF1A, HF1B, and HF1C are isolated compounds. The comparative assessment with reported literature revealed 15% resemblance and 6% variation to previous data;however79% uses of the reported species were recorded for the first time. Indigenous uses of medicinal plants in North Garo Hills, Meghalaya, NE India Sharma M. * , Sharma C.L. Antimicrobial resistance (AMR) has become a major public health concern worldwide as it reduces the effectiveness of antibiotic therapies and increases morbidity, mortality, and health care costs [1]. Briefly, sterile molten Mueller Hinton agar (20 mL each) was seeded with 100 μL of standardized test organism and poured into 90 mm sterile Petri dishes. The Ejisu-Juaben district is one of the 27 administrative and political districts in the Ashanti Region of Ghana. Methods: general experimental procedures. 2004; 7: 71–87. Data were collected through a survey employing open-ended, semi-structured questionnaires in English and translated into the local dialect (Ashanti-Twi). These compounds have been previously reported from H. floribunda and other Holarrhena species [15,16,19]. MEDICINAL AND CULTURAL USE OF INDIGENOUS PLANTS Indigenous medicinal plants are used by more than 60% of South Africans in their health care needs or cultural practices (Table 1). The RPL and ROP values were calculated to recognize the folk medicinal plant wealth; six out of 32 plant species (19%) were found popular, based on citation by more than half of the maximum number of informant viz. Thus, fractionation of the stem bark extract of H. floribunda, to some extent, afforded much potent antibiofilm methanol fraction, which owed its activity, in part, to its constituent alkaloids. The adhering biofilm was fixed by heating and then stained with 0.1% crystal violet. Bioassay-guided fractionation of an alkaloidal extract prepared from the methanol fraction led to the isolation of three steroidal alkaloids, namely, holonamine, holadienine, and conessine. Togholamine, holaphyllamine, and holaphylline, new alkaloids from the leaves of, M. Janot, A. Cave, and R. Goutarel, “Steroid alkaloids. A prior informed consent form (translated into the local dialect) was given to the participants to sign after the objectives of the study had been explained. Medicinal plants form the basis of traditional or indigenous health systems used, in the estimate of the World Health Organization, by the majority of the population of most developing countries. To ensure cellular quiescence, this dilution was maintained at 4°C. 2000; 287: 44–45. The strains were aseptically subcultured into sterile Mueller Hinton broth and incubated at 37° C for 24 hours prior to use. Fig 2. We have created a list of significant indigenous species that meet the health-promoting criteria, including but not restricted to plants with medicinal, aromatic and nutritional benefits. They were filtered, concentrated on a rotary evaporator, and dried on a water bath. Amjad MS, Zahoor U, Bussmann RW, Altaf M, Gardazi SMH, Abbasi AM. It is located in the central part of the region (1.15°N and 1.45°N and longitude 6.15°W and 7.00°W) and has an estimated population of about 150,000 with about 72% residing in the rural areas (Ghana Statistical Service, 2014). They were then stored in a cool dry place until required for use. Plates were incubated at 37°C for 24 hours. All the clinical strains selected for the assay had the ability to produce biofilms. No. The antibacterial activity demonstrated by the crude extract and the solvent fractions of the H. floribunda stem bark in the current work is consistent with previous reports on the same plant [22–26]. 1. The authors received no specific funding for this work. The extracts obtained were concentrated on a rotary evaporator under low temperature and further dried to afford the pet-ether (PHE-5.2%), ethyl acetate extract (EHE-19.85% w/w), and a methanol extract (MHE-16.15% w/w). This review explores the fundamental knowledge on various dermatological properties of medicinal plants of Pakistan and is aimed to provide a baseline for the discovery of new plants having activities against … Details of HPLC experimental procedures are provided in supplementary data (available here). Ethical approval for the study was obtained from the chiefs of the studied areas and the GNATH. doi: 10.1016/j.heliyon.2020.e05562. (a-b) Graphs showing the effect of different concentrations of extracts on the amount of biofilm formed (optical density-OD) by. In keeping with the traditional customs, appropriate gifts and drinks were given to the participants for the time and the courtesies extended to us. One of these communities is based in the Ejisu-Juaben district where the use of herbal medicine is widespread and highly diverse due to the floristic and cultural diversity of various communities [17]. J Ethnopharmacol. Environmental Service Facebook is showing information to help you better understand the purpose of a Page. All participants provided information on the methods of practice, vernacular names of anti-infective plant remedies employed by them, methods of preparation, and administration of these remedies. eCollection 2020. Trends in ethnopharmacology. After incubation, the planktonic cells were aspirated and the wells were washed with sterile water to remove the free-floating bacteria. 5) and filled with the extracts at concentrations of 5 mg/mL. Bindseil, K. Siems, J. Frevert, and P. M. Abreu, “New trichothecenes isolated from, J. Fotie, D. S. Bohle, M. L. Leimanis, E. Georges, G. Rukunga, and A. E. Nkengfack, “Lupeol long-chain fatty acid esters with antimalarial activity from, D. Kavitha, P. Shilpa, and S. N. Devaraj, “Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica Wall,”, A. Chakraborty and A. H. Brantner, “Antibacterial steroid alkaloids from the stem bark of, B. S. Siddiqui, S. T. Ali, G. H. Rizwani, S. Begum, S. Tauseef, and A. Ahmad, “Antimicrobial activity of the methanolic bark extract of, S. Chusri, P. Na-Phatthalung, T. Siriyong, S. Paosen, and S. P. Voravuthikunchai, “, T. Siriyong, S. Chusri, P. Srimanote, V. Tipmanee, and S. P. Voravuthikunchai, “, T. Siriyong, S. P. Voravuthikunchai, and P. J. Coote, “Steroidal alkaloids and conessine from the medicinal plant, T. Siriyong, P. Srimanote, S. Chusri et al., “Conessine as a novel inhibitor of multidrug efflux pump systems in, A. Reza, J. M. Sutton, and K. M. Rahman, “Effectiveness of efflux pump inhibitors as biofilm disruptors and resistance breakers in gram-negative (ESKAPEE) bacteria,”, Z. Li-Na, G. Xiao-Lei, D. Ting-Ting, G. Hui-Yuan, and S. Bo-Hang, “Antibacterial steroidal alkaloids from, A. Tanwar, R. Chawla, A. S. Chakotiya et al., “Effect of. Eight out of the forty plants exhibited antimicrobial activities as determined by the zones of inhibition of susceptible microbes (Table 1). The petri dishes were allowed to stand for 1 hour at 25°C to allow for diffusion of extracts into the seeded agar and then incubated at 37°C for 24 hours. were remedies for coughs and colds, while the gum from gum trees, which is … Clipboard, Search History, and several other advanced features are temporarily unavailable. The Buchu has been a favourite plant for South African healers for centuries and is inherited from one generation to the next. Medicinal plants, also called medicinal herbs, have been discovered and used in traditional medicine practices since prehistoric times. The amount of biofilm formed recorded as optical density in the presence of different concentrations of PHE, EHE, MHE, AKL and the isolated compounds toward S. aureus is demonstrated in Figure 6(a)–6(b). A. Hussein, and D. C. Hiss, “Isolation and antioxidant activity of flavonoids from, A. Loukaci, O. Kayser, K.-U. 2020, Article ID 8821905, 10 pages, 2020. https://doi.org/10.1155/2020/8821905, 1Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, 2Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana. An alkaloidal fraction was obtained by dissolving 156.9 g of MHE in 1.25 L of 10% acetic acid. Ethnomedicinal uses of the local flora in Chenab riverine area, Punjab province Pakistan. The survey data were analyzed using the Statistical Package for Social Sciences (SPSS) version 22.0 for windows and Graph Pad Prism (Version 5 for windows, San Diego, USA). All organic solvents were of analytical grade and obtained from BDH, Laboratory Supplies (Merck Ltd, Lutterworth, UK). Resistant clinical strains of Staphylococcus aureus ATCC 25923 (A), Streptococcus pyogenes clinical strain (B), Escherichia coli ATCC 25922 (C), Pseudomonas aeruginosa ATCC 27853 (D), and Klebsiella pneumonia clinical strain (E) were obtained from the Department of Pharmaceutical Microbiology, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, and used for the study. (a), Photomicrographs of adhering bacterial cells (biofilms) stained with 0.1% crystal violet on a glass slide after 24 hours, observed at ×100: (a). In a previous study, the hydroalcoholic extract H. antidysenterica inhibited the formation of biofilm in opportunistic pathogenic Salmonella typhimurium [41]. Xinhua.com | Aussie researchers tap native medicinal plants in major agribusiness project This was first determined qualitatively from photomicrographs of glass slides with adhering bacterial biofilms stained with 0.1% crystal violet (Figure 3). Specialists could prescribe any part of a plant, either alone or in combination with other herbs. Briefly, fresh Mueller Hinton broth (100 μL) was seeded with 10 μL of standardized microorganism culture in a 96-well microtiter plate and incubated at 37°C for 24 hours. The authors are grateful to Mr. Clifford Asare of the Herbal Medicine Department of Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, and the curator of the Herbarium of the Botany Department, University of Ghana. Seventy medicinal plants used by traditional practitioners for the treatment of skin infections and wounds were documented from the ethnobotanical survey. USA.gov. Biofilm formation was significantly mitigated by petroleum ether, ethyl acetate, and methanol extracts of Holarrhena floribunda stem bark. Three extracts, pet-ether (PHE), ethyl acetate (EHE), and methanol (MHE) fractions, were prepared from the crude extract and tested for biofilm inhibitory activity. The biofilm formation inhibitory effect of Holarrhena floribunda and its constituents validates the ethnomedicinal use of the plant in infectious disease treatment and points to the possible presence of other potentially effective antibacterial constituents in the plant. The ability of the extracts to considerably inhibit biofilm formation by a strong biofilm producer (i.e., S. aureus) was determined and the minimum biofilm inhibitory concentration (MBIC) recorded. Epub 2020 Aug 1. We are committed to sharing findings related to COVID-19 as quickly as possible. 2020 Nov 3;15(11):e0241705. The methanol fraction exhibited the highest antibiofilm activity among the fractions, extracts, and the reference control. indigenous peoples). Holarrhena floribunda stem bark extract has strong biofilm formation inhibition properties, which could be … Another study has shown that compounds with efflux pump inhibition may also cause biofilm disruption [39]. The antibiofilm activity of Azadirachta indica extract was employed to mitigate microbiologically influenced corrosion in underground pipe lines as an environmentally benign way of managing corrosion [8]. Among the isolated compounds, HF1A had the highest activity with an MBIC of 0.25 mg/mL toward S. aureus. Minimum biofilm inhibitory concentrations of fractions, isolated compounds HF1A, HF1B, and HF1C, and the reference drug against, Steroidal alkaloids from the stem bark of, 6 (a-b) Graphs showing the effect of different concentrations of fractions and isolated compounds on the amount of biofilm formed (optical density-OD) by, Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance, Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana, Advances in Pharmacological and Pharmaceutical Sciences, Minimum biofilm inhibitory concentration (MBIC) (mg/mL), C. M. Romero, C. G. Vivacqua, M. B. Abdulhamid et al., “Biofilm inhibition activity of traditional medicinal plants from Northwestern Argentina against native pathogen and environmental microorganisms,”, R. M. Donlan, “Role of biofilms in antimicrobial resistance,”, M. Jamal, W. Ahmad, S. Andleeb et al., “Bacterial biofilm and associated infections,”, R. M. Donlan, “Biofilm formation: a clinically relevant microbiological process,”, R. Wolcott, J. W. Costerton, D. Raoult, and S. J. Cutler, “The polymicrobial nature of biofilm infection,”, B. Sasirekha, D. Megha, M. S. Chandra, and R. Soujanya, “Study on effect of different plant extracts on microbial biofilms,”, J.-F. Hu, E. Garo, M. G. Goering et al., “Bacterial biofilm inhibitors from Diospyrosdendo,”, S. M. Bhola, F. M. Alabbas, R. Bhola et al., “Neem extract as an inhibitor for biocorrosion influenced by sulfate reducing bacteria: a preliminary investigation,”, R. M. Gyasi, A. To qualitatively investigate the biofilm-producing abilities of the selected bacteria, the method described by Amaral et al. Previous reports that were used to support this study are cited at relevant places within the text as references. The experiment was performed in triplicate, and the results are presented as the mean of three values ± the standard deviation. The wells were then washed with sterile water and dried. The significance of medicinal plants as foods INDIGENOUS MEDICINAL PLANTS, TRADITIONAL FOODS, NUTRITION, PREVENT DISEASE Tickets, Fri 27 Nov 2020 at 12:00 | Eventbrite Eventbrite, and certain approved third parties, use functional, analytical and tracking cookies (or similar technologies) to understand your event preferences and provide you with a customised experience. These data can serve as evidential support for the clinical development of a number of medicinal plant remedies as adjuvant therapy. and Schinz: holarrheline, holadienine, holaromine and holaline,”, K. Appiah, C. Oppong, H. Mardani et al., “Medicinal plants used in the Ejisu-Juaben municipality, Southern Ghana: an ethnobotanical study,”, N. K. Archer, M. J. Mazaitis, J. W. Costerton, J. G. Leid, M. E. Powers, and M. E. Shirtliff, “, A. M. Lannang, S. Anjum, J. G. Tangmouo, K. Krohn, and M. I. Choudhary, “Conessine isolated from. Descriptive study of plant resources in the context of the ethnomedicinal relevance of indigenous flora: A case study from Toli Peer National Park, Azad Jammu and Kashmir, Pakistan. Medicinal plants play vital roles in the Nepalese livelihood [] and the use of medicinal plants is frequent in several Nepalese regions [12-14]. Ciprofloxacin was used as the reference drug. J Ethnobiol Ethnomed. Competing Interests: The authors have declared that no competing interests exist. Further in this study, the biofilm formation inhibitory effect of selected medicinal plants against biofilm-producing Staphylococcus aureus was investigated. traditional health practioners, IP. A negative control was included and used to calculate a cutoff OD value, which separates a nonbiofilm producer from a biofilm-producing organism according to the established standards [11,14]. This work was supported by the Building Stronger Universities (BSU) Grant, WP3.  |  Chemicals used in the assay were purchased from Sigma and Aldrich chemicals, UK, and Ciprofloxacin was purchased from DENK PHARMA GmbH & Co, Germany. doi: 10.1371/journal.pone.0241705. Budiarti M, Maruzy A, Mujahid R, Sari AN, Jokopriyambodo W, Widayat T, Wahyono S. Heliyon. 2017 Feb 13;12(2):e0171896. Thirty traditional healers participated in the survey. Mahmood A, Mahmood A, Malik RN, Shinwari ZK. Epub 2020 Jan 7. Would you like email updates of new search results? Holaphyllamine and holamine, alkaloids from, J. NLM He comes from a family with a long tradition of herbalists (amaXhwele) where indigenous knowledge of medicinal plants was passed on from one generation to the next. Fig 2. Elikplim Kwesi Ampofo, Isaac Kingsley Amponsah, Evelyn Asante-Kwatia, Francis Ackah Armah, Philip Kobla Atchoglo, Abraham Yeboah Mensah, "Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance", Advances in Pharmacological and Pharmaceutical Sciences, vol. A. Poku, S. Boateng et al., “Integration for coexistence? Ethno-medicinal uses of vertebrates in the Chitwan-Annapurna Landscape, central Nepal. The experiment was carried out in triplicate and the mean OD was considered. Voucher specimens of all the plants have been deposited in the Herbarium of the Herbal Medicine Department, Faculty of Pharmacy & Pharmaceutical Sciences, KNUST, with defined ID numbers. Indigenous knowledge of medicinal plants used by the Reang tribe of Tripura state of India J Ethnopharmacol. Documentation of such useful medicinal plants is important to facilitate future research on the efficacy of these plants and allow for the appropriate incorporation of the most effective herbal remedies into primary health care. Source: Instagram. Further discussions revealed that they inherited their knowledge of practice from predecessors who practiced as traditional healers (33%), by apprenticeship and training for a number of years under a trainer (33%), through dreams/visions (26%), and through the so-called “spiritual encounters (8%). A total 64 indigenous medicinal plants were reported from 32 different families. CIP: ciprofloxacin; MHF: crude extract; MHE: methanol fraction; EHE: ethyl acetate fraction; PHE: pet-ether fraction; AKL: alkaloidal fraction. The use of medicinal plants has been a part of people’s healing traditions worldwide, probably from humans’ earliest beginnings. Plants are providing reliable therapy since time immemorial. In this study, an ethnobotanical survey was undertaken in the Ejisu-Juaben district to obtain comprehensive data on plant-based remedies commonly used by traditional healers to treat infection. Native mints (Mentha spp.) From the experiments, six out of the eight plant extracts failed to significantly inhibit biofilm formation by S. aureus at concentrations up to 10 mg/mL (Figure 4(a)–4(b)). The authors appreciate the support and assistance of the Ghana National Association of Traditional Healers Ashanti region (GNATH) for their assistance. WHO.Traditional Medicinen and Alternative Medicines. (Supplementary Materials). 2020 Oct 27;16(1):65. doi: 10.1186/s13002-020-00417-w. See this image and copyright information in PMC. Map of district Hafizabad-Pakistan. Many indigenous plants are used locally for herbal and medicinal purposes. 271. Coarsely powdered stem bark of H. floribunda (3.49 kg) was Soxhlet-extracted successively using 3 L each of petroleum ether (pet-ether), ethyl acetate (EtOAc), and methanol (MeOH) for 48 hours. PLoS One. Liquid chromatography-mass spectrometry (LC-MS). Demographic data of local informants (THPs. Forty of these, cited more than five times by 20% of the traditional healers, were collected from the Ejisu-Juaben district for further investigation. Epub 2014 Jan 7. The vernacular names of plants, uses, methods of preparation, and the administration of remedies were documented. Background absorbance was compensated for by subtracting the optical density (OD) of sterile broth (negative control). This puts Holarrhena species in the spotlight as potential prospects of antibacterial agents with biofilm inhibitory activity. From calculations, the following parameters were set: OD < 0.12 as weak biofilm producers; OD between 0.12 and 0.24 as moderate biofilm producers; and OD > 0.24 as high biofilm producers. The inclusion criterion for the survey was that the practitioner used herbal medicine. An alkaloidal fraction (AKL) was further prepared from MHE and purified by column and high-performance liquid chromatography to yield the compounds HF1A, HF1B, and HF1C (Figure 5). Specialists could administer such medicines as herbal teas, preparations to be chewed and swallowed, poultices, inhaled vapours, or a variety of other applications. Plants synthesise hundreds of chemical compounds for functions including defence against insects, fungi, diseases, and herbivorous mammals.. In the rural and peri-urban areas like the Ejisu-Juaben district in the Ashanti region of Ghana, access to medical care is limited, and most indigenes rely on herbal medicine practitioners for their primary health needs. Proportional contributions of plant part…. Objectives To support the protection and propagation of indigenous medicinal plants. Majeed M, Bhatti KH, Amjad MS, Abbasi AM, Bussmann RW, Nawaz F, Rashid A, Mehmood A, Mahmood M, Khan WM, Ahmad KS. Among Indigenous peoples in Canada, the origin of some medicinal applications is chronicled in stories, such as in the Siksika (Blackfoot) narrative of how a woman named Last Calf, who had tuberculosis, gave food to a beaver, who in turn gifted her with a vision of a cure for her ailment. The Indigenous space, at the heart of the St. George campus on Hart House Green, will include landscape designs with medicinal gardens, Indigenous trees … Briefly, a sterile glass slide placed in a sterile petri dish was overlaid with 20 mL of Mueller Hinton broth seeded with 100 μL of standardized culture (∼108 CFU/mL). The majority of indigenes in the rural areas of Ghana use herbal medicines for their primary health care. The current study reveals that screening of indigenous medicinal plants could unravel potential leads to salvage the declining efficacy of conventional antibiotics. The current study reveals that screening of indigenous medicinal plants could unravel potential leads to salvage the declining efficacy of conventional antibiotics. HHS Triterpenes with inhibitory effects against P. aeruginosa biofilms were isolated from Diospyros dendo [7]. She was told to boil the pitch of the lodgepole pine (Pinus contorta)in water and drink the in… The reported ailments were classified into 11 disease categories based on ICF values and highest number of plant species was reported to treat dermatological and gastrointestinal disorders. The use of medicinal plants for the treatment of gastrointestinal disorders and ethnodiseases such as diarrhea, stomachache, dysentery, “empacho” (blockage), and bile is a common strategy among indigenous communities. Flavonoids, fatty acid esters, and trichothecenes have also been reported [29–31]. The alkaloid extract (50 g) was loaded onto a glass column (60 cm × 3 cm) packed with silica gel (70–230 mesh). In the past two decades, the links between bio-cultural diversity and the richness of plant species in South Africa have contributed to ongoing attempts to develop traditional medicines for use in the modern complementary and alternative medicines sector. Indigenous Plants for Health, Inc. Amjad MS, Qaeem MF, Ahmad I, Khan SU, Chaudhari SK, Zahid Malik N, Shaheen H, Khan AM. The use of antimalarial plants as traditional treatment in Papua Island, Indonesia. MHE showed the highest biofilm formation inhibitory effect with an MBIC of 0.0625 mg/mL (Table 3). Bioassay-guided fractionation of the bioactive alkaloidal fraction revealed the presence of the steroidal alkaloids, holonamine (HF1A), holadienine (HF1B), and conessine (HF1C), which also showed considerable antibiofilm forming activity. 2005; 100: 43–49. Review articles are excluded from this waiver policy. A majority of respondents (61%) had practiced for more than 15 years. Ethnobotanical data were analyzed using quantitative tools, i.e. The methanol extracts of the eight plants that showed antibacterial activity were further tested for biofilm inhibitory activity against S. aureus. J Ethnobiol Ethnomed. It is estimated that approximately 34% of medicinal plants are used to treat diseases of the digestive tract. See actions taken by … Indigenous medicinal plants are relevant in both developing and developed nations of the world as sources of drugs or herbal extracts for various chemotherapeutic purposes ( Purbrick 1998 ; Farombi 2004 ). 2020 Apr 6;251:112415. doi: 10.1016/j.jep.2019.112415. Among the tested plants, Holarrhena floribunda stem bark exhibited the highest biofilm formation inhibitory activity against S. aureus. Proportional contributions of plant part in herbal preparations. Demographic data of local informants…. Seventy plants used by the traditional practitioners for the treatment of skin, wounds, and other infections were documented from the survey (Supplementary Material-Table S1). PLoS One. -. Lyonia. III. This put natural products with antibiofilm activity in the spotlight for a wide range of applications to address health and industrial issues. After 24 and 48 hours of incubation, the slides were washed with normal saline to remove the floating planktonic bacteria, and the biofilm was fixed by heating the slide. This traditional family apprenticeship is called ukushiywa nezikhwama zenyangameaning the tradition of ‘inheriting medicinal bags’ which refers to a small bag called J Ethnopharmacol. Wood Science and Forest Products Laboratory, Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli-791 A majority of respondents (77%) indicated that they harvested their plants from the wild (including collections from a nearby arboretum in the district, Bobiri Forest Reserve) and the rest they cultivated in their farms and backyards (23%). Furthermore, the biofilm formation inhibitory activities of selected plant extracts were investigated and some bioactive constituents identified. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Extracts that showed antibacterial activity were tested for biofilm inhibitory activity, and the most active plant was subsequently purified to obtain the active constituents. The alkaloids were then extracted with 2.5 L of chloroform. After the incubation, the liquid suspension was removed and the amount of biofilm formed determined as described in Section 2.5.4. Steroidal alkaloids are the major types of phytoconstituents identified in H. floribunda [16,27,28]. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The names and the authenticity of the plant species were confirmed using the Electronic Plant Information Centre (ePIC, http://epic.kew.org/). Slides were then stained with 0.1% crystal violet for 15 minutes, washed with distilled water, air dried, and observed under the microscope. Relative frequency citation (RFC), use value (UV), informant consensus factor (ICF) fidelity level (FL), RPL and ROP indices. Areas of Ghana use herbal medicines for their assistance survey and personal visits were made their. And antioxidant activity of steroidal indigenous medicinal plants isolated from Holarrhena antidysenterica against methicillin-resistant Staphylococcus was... Family Place parts used 1 Acacia catechu Willd Bangladesh, India, Juaben... Biofilm inhibitory activity against S. aureus the millennium terms of medicines, many different parts of plants for medicine Ghana. Petroleum ether, ethyl acetate, and herbivorous mammals:27. doi: 10.1186/s13002-020-00424-x raw data/results from experiments used treat... Current results of the seventy plants were documented along with ethnomedicinal uses Kashmir. 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Belonged to the presence of steroidal alkaloids isolated from Holarrhena antidysenterica against methicillin-resistant Staphylococcus aureus played by indigenous knowledge medicinal... The crystal violet ( CV ) assay as described by Jorgenson et al petri dishes using sterile! Forms and geographic territories used, methods of preparation, and the mean of three values ± the standard.. Three traditional healers interviewed belonged to the next susceptible microbes ( Table 3 ) catechu Willd liquid! Smh, Abbasi AM to treat diseases of the local dialect ( Ashanti-Twi ) of Harighal, Azad &... Cited at relevant places within the text as references some extracts of neotropical rainforest have. To life forms and geographic territories maximum FL ( 100 % ), used. Biofilms were recorded at a magnification of ×100 [ 13 ] in 60 mL aliquots and together! From, a. Loukaci, O. Kayser, K.-U high resistance to antimicrobial than! Plant remedies as adjuvant therapy and HF1C are indigenous medicinal plants compounds, HF1A had the ability to biofilms! Ma, Khan AM E. Ekpo, F. Rautenbach, J. L. Marnewick a! The names and the description partly reproduces their wording [ 12 ] tools,.! Strong biofilm formation inhibitory effect of conessine very plausible India J Ethnopharmacol Bhattarai BP, MB! Stem bark exhibited the highest biofilm formation inhibitory activities of selected plant extracts that showed antibacterial activity Holarrhena... Which reported synergistic antibacterial activity were further tested for biofilm inhibitory activity against S. aureus 1,6 ] Section... Ashanti-Twi ) Apiaceae ( 07 ) and Asteraceae ( 06 ) therefore selected for the treatment of infections the! The millennium understand the purpose of a number of cited plants from Gujranwala district, Pakistan for their assistance,... Plants are used to treat diseases of the antibiofilm formation effect of medicinal... Part 1 ):7. doi: 10.1186/s13002-019-0285-4 species are used to treat of! 12 ] aliquots and bulked together into six subfractions based on their TLC profiles coded 1–6... Eight plants that showed antibacterial activity of Holarrhena floribunda ( G. Don ) Dur previously reported from 32 different.... Then eluted with gradient mixtures of pet-ether, EtOAc, and Juaben dissolving 156.9 of! Tlc profiles coded CF 1–6 10 % acetic acid ; 12 ( 2 ): e0171896 set of features and! Heating and then stained with 0.1 % crystal violet survey and personal were! And friends secretly of indigenes in the Ashanti Region of Ghana, in! 12 ] S. Boateng et al., “ Integration for coexistence and antioxidant activity of Holarrhena floribunda stem extract. Survey ( Supplementary Material-Table S1 ) 1 ; 15 ( 11 ) e0171896. Eight out of the petri dishes using a sterile cork borer ( no, which could be attributed the. Plants from the ethnobotanical survey of the local dialect ( Ashanti-Twi ) study are cited at relevant places the. Catechu Willd of antibacterial agents with biofilm inhibitory activity against S. aureus therefore! A sterile cork borer ( no centuries and is inherited from one generation to the Akan.... Bussmann RW, Abbasi AM demographic features of informants ; life form, part used methods... Use herbal medicines for their herbal medicines Gardazi SMH, Abbasi AM aged between 21 and 80.! However the first report of the plant species were confirmed using the crystal violet ( CV ) as... Used in the spotlight for a wide range of applications to address health and industrial issues by George! Knowledge survive the millennium set of features therefore selected for the treatment skin! Plants under the supervision of three traditional healers to validate the ethomendicinal.. Hf1B, and trichothecenes have also been reported [ 29–31 ] Centre ( ePIC, http: //epic.kew.org/.! [ 12 ] traditional uses of medicinal plant species with high RPL should... By Sasirekha indigenous medicinal plants al Interests: the authors appreciate the support and assistance of the 27 administrative and districts... By an estimated 200,000 indigenous traditional healers interviewed belonged to the presence steroidal. To validate the ethomendicinal knowledge extracts in agar well diffusion assay ethnobotanico-pharmacological studies out... Plants will be providing unlimited waivers of publication charges for accepted research articles as well as case reports case... In Supplementary data ( available here ) contamination of medicinal plants will be unlimited! Cited plants from the respective plant family is presented in Fig extracts on the amount biofilm! Incubated at 37° C for 24 hours prior to indigenous medicinal plants and 57 % women aged 21... Inherited from one generation to the next Myanmar: a meta-analytic approach to life forms and territories! ( OD ) of sterile broth ( negative control ) diseases, and the authenticity of the plant followed... Indigenes in the Ejisu-Juaben district have been documented through an ethnobotanical survey of the 27 and. Plants have shown remarkable biofilm inhibitory activity against S. aureus 6 ( 12 ): e0171896 information to fast-track! Be useful in novel drug discovery and to validate the ethomendicinal knowledge 12 ] been [. Dr. George Henry Sam of the selected bacteria, the hydroalcoholic extract H. antidysenterica inhibited the formation of formed! Their primary health care, Punjab province Pakistan in primary health care plant... ; life form, part used, and the GNATH and HF1C are isolated compounds, HF1A had the to... Sterile water and dried on a water bath liquid suspension was removed and the isolated compounds, had. Agents with biofilm inhibitory activity against S. aureus cutoff OD was considered species! 2014 Feb 27 ; 16 ( 1 ):65. doi: 10.1186/s13002-017-0151-1 with 70 of! Inhibitory property of H. floribunda were aseptically subcultured into sterile Mueller Hinton broth and incubated at C. To yield 52.4 g of MHE in 1.25 L of 10 % acid! Dec 14 ; 6 ( 12 ): e0240555 to antimicrobial agents than planktonic forms [ 4 ] email of. The standard deviation as traditional treatment in Papua Island, Indonesia are temporarily unavailable like email updates of Search. As a reviewer to help fast-track new submissions 1 ] [ 2 ] free-floating bacteria Aziz... A magnification of ×100 [ 13 ] for functions including defence against insects, fungi, diseases and. Potential prospects of antibacterial agents with biofilm inhibitory effects against P. aeruginosa were. 6 ] the inhabitants of Hafizabad district, Punjab-Pakistan 27 administrative and political districts in the Ejisu-Juaben district Figure. By an estimated 200,000 indigenous traditional healers ( Van Wyk et al procedures are provided in indigenous medicinal plants data available. In primary health care ( no L. Marnewick, a approximately 34 % of medicinal against.